One step screening of retroviral producer clones by real time quantitative PCR
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چکیده
منابع مشابه
A one-step method for quantitative determination of uracil in DNA by real-time PCR
Uracil may occur in DNA due to either cytosine deamination or thymine replacing incorporation. Its quantitative characterization is important in assessing DNA damages in cells with perturbed thymidylate metabolism or within different DNA segments involved in immunoglobulin gene diversification. The archaeal DNA polymerase from Pyrococcus furiosus binds strongly to the deaminated base uracil and...
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Those who have worked in the field of quantitative polymerase chain reaction (PCR) since the early 1990s have accepted many of the tedious aspects of the early assays as routine. Difficulties associated with early quantitative PCR techniques included: (i) ensuring that the PCR was within the linear range of amplification (that portion where the PCR signal is directly proportional to the input c...
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Background: Acute myeloid leukemia (AML) is one of myeloid malignancies which the risk increases with age increment. It is categorized based on genetic aberrations. Some of these genetic disorders can determine minimal residual diseases (MRD) and prognosis of AML patients. Wilms tumor (WT1) over expression is found in AML patients. The aim of this study was to determine the frequency of WT1 ove...
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ژورنال
عنوان ژورنال: The Journal of Gene Medicine
سال: 1999
ISSN: 1099-498X,1521-2254
DOI: 10.1002/(sici)1521-2254(199909/10)1:5<352::aid-jgm57>3.0.co;2-i